A real-time amplification plot was generated using the normalized reporter signal (Rn). The PCR product yield or ΔRn was defined as the Rn minus the baseline signal established in the first few ...

Thanks to its ability to gather data in the exponential growth phase of PCR, real-time PCR finds use in several novel applications where traditional PCR would not have been as effective.

The largest application of PCR and realtime PCR testing resides within clinical diagnostics, a trend that is expected to progress further, while the end-user segment analysis shows clinical ...

By matching the right master mix reagent, staple chemical components necessary for performing real-time PCR reactions, with the kind of application for which the reagent was designed, researchers can ...