Danielle is a Senior Editor at The Scientist. She has a background in neuroscience. Her science communication experience spans journalism, scientific publishing, and science education and outreach.

The methods used to deliver Cas9/sgRNA into the target cells strongly influence its off-target effect. Generally, Cas9/sgRNA is delivered via viral transduction, ribonucleoprotein (RNP ...

The Cas9 protein is the most widely used by scientists. This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it ...

At the heart of this technology is the Cas9 protein, often likened to molecular scissors, capable of cutting strands of DNA at specific locations dictated by a single guide RNA. With this ...

“Our screening platform significantly accelerates the screening processes and is applicable to a variety of libraries, including CRISPR/Cas9 sgRNA, shRNA, and even small compounds,” said Asako ...