Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to select ...

Hence for this data, normalizing with total RNA is essentially as good as normalizing with the single best reference gene (SD ~0.05). Normalizing with the seven best reference genes, however ...

The normalized expression data for hOGG1 gave the expected or opposite conclusions when normalized to the most or least stable reference gene targets, respectively (Figure 1A) 6.

This article describes how researchers from Queen’s University Belfast investigated the expression stability of 8 candidate reference genes in cerebellum and medial temporal gyrus of 6 AD, 6 PD, 6 DLB ...

Please use one of the following formats to cite this article in your essay, paper or report: APA. LGC Biosearch Technologies. (2024, August 28). How to build qPCR assays like a pro.