In a new study published in Nature titled, “Custom CRISPR-Cas9 PAM variants via scalable engineering and machine learning,” researchers from Massachusetts General Hospital (MGH) and Harvard ...

本文聚焦于 CRISPR-Cas9 在微生物细胞工厂的应用。介绍了其作用机制和应用，探讨如何优化该技术，如降低脱靶效应、提高靶向效率，为推动此技术在微生物领域的发展提供全面视角，助力相关 ...

然而，这类酶存在一些问题，比如脱靶编辑风险增加 3,5,6 。为了实现 Cas9 酶的可扩展重编程，研究人员将高通量蛋白质工程与机器学习（ML）相结合，开发出更适合特定靶点的定制编辑器。

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Apr. 17, 2025 — A research team has decoded the genome of historic potato cultivars and used this resource to develop an efficient method for analysis of hundreds of additional potato ...

The flexibility of CRISPR/Cas9 editing is severely constrained by the requirement that the modification site is located in close proximity to the landing sequences. Here, authors develop a ...

Apr. 4, 2025 — A novel cell therapy approach using cord blood-derived natural killer (NK) cells pre-complexed with AFM13, or acimtamig, a CD30/CD16A bispecific antibody, was safe and generated ...