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At the heart of this technology is the Cas9 protein, often likened to molecular scissors, capable of cutting strands of DNA at specific locations dictated by a single guide RNA. With this ...
Lentiviruses with each sgRNA were produced and transduced to Cas9-expressing cell lines. In the cell fitness assay, puromycin selection (Thermo Fisher Scientific, A1113803) was performed to confirm ...
Each of these presents its own advantages and challenges. A Cas9 RNP complex consists of the Cas9 protein and a sgRNA. mRNA-based cargoes encoding Cas9 only require delivery to the cytosol for ...
Most recently, CRISPR interference (CRISPRi) has been developed as an emerging technology that exploits the catalytically inactive Cas9 (dCas9) and single-guide RNA (sgRNA) to repress ...
Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9
These results indicated that (1) individual sgRNAs that cleave quiescent HSV genomes efficiently can be identified and (2) SaCas9/sgRNA-induced indel mutations lead to loss-of-function mutations in ...
We used these sgRNAs to associate with the Cas9 endonuclease to cut a 732 bp HNF4α promoter fragment. The results indicated that the target DNA could be digested by all sgRNAs (Figure 1b), indicating ...
Then, using a virus as a delivery vehicle, he programmed each cell with a different single-guide-RNA (sgRNA) sequence of genetic code. In combination with the Cas9 enzyme, the sgRNA codes—about 20 ...
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