We subsequently evaluated the binding affinity of LARP7 mutants to 7SK snRNA by using RNA immunoprecipitation (RIP)-qPCR. The results showed that the 49KA mutant almost completely lost the binding of ...

The total RNA was extracted by TRIzol, and reverse transcription was conducted for the subsequent qPCR assay. Gel Electrophoresis was performed to validate the products of RIP-qPCR. Each sample of the ...

5µl of the cDNA was used for SYBR Green qPCR Master mix (Bio-Rad 1725275 ... partner repository with the dataset identifier PXD047402. RNA-seq and RIP-seq data were submitted to GEO under accession ...

When a hurricane approaches the U.S., forecasts for high winds and intense rain often grab the attention, but rip currents far away from the landfall location also present a deadly hazard.

On April 2, 2025, the Trump Administration declared “Liberation Day” in the United States and rolled out a reciprocal tariff regime to “restore national and economic security.” Evidence ...

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Well-known to followers of the blogosphere and seekers of intelligent, percipient commentary on public policy, Kevin Drum passed away Friday after a long battle with multiple myeloma, a cancer of ...

Roula Khalaf, Editor of the FT, selects her favourite stories in this weekly newsletter. Who are the true pioneers of quantitative finance? Even for us here at FT Alphaville, it’s a difficult ...

Gov. Tim Walz speaks at Roosevelt High School in Des Moines, Iowa alongside Iowa Democratic Party Chair Rita Hart on Friday. From an auditorium stage at a Des Moines high school on Friday ...