ATP assays are procedures that can measure cell viability based on detection of ATP. Learn how ATP assays work and how to choose the right assays for your needs.

Detect and quantify ATP in biochemical and cell viability assays with the ATP Determination Kit. This luminescence-based ATP assay utilizes the luciferase-luciferin reaction, offering exceptional sensitivity to detect as little as 0.1 picomole of ATP using a luminometer or microplate reader.

Jul 6, 2022 · ATP testing helps to detect the ATP produced by live microorganisms like bacteria from various surfaces and water, which helps assess the hygiene in the food industry and test the water quality quickly. But ATP testing does not help detect viruses as they do not produce ATP.

May 1, 2013 · The assay methods covered include the use of different classes of colorimetric tetrazolium reagents, resazurin reduction and protease substrates generating a fluorescent signal, the luminogenic ATP assay, and a novel real-time assay to monitor live cells for days in culture.

The ATP test is a process of rapidly measuring actively growing microorganisms through detection of adenosine triphosphate, or ATP. ATP is a molecule found in and around living cells, and as such it gives a direct measure of biological concentration and health.

The CellTiter-Glo 2.0 Assay is a simple, luminescence-based method to detect viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells.

The Agilent Seahorse ATP Real-Time rate assay is the only assay that quantifies the rate of adenosine triphosphate (ATP) production from glycolysis and mitochondria simultaneously using label-free technology in live cells.

In this article, we will specifically focus on the ATP bioluminescence assay, which uses the relationship between ATP, luciferin and luciferase to help determine the number of live cells. What is the ATP bioluminescence assay? How does the ATP bioluminescence assay work? Luciferase Reaction. What applications use the ATP bioluminescence assay?

The ATPase assay is a membrane assay that indirectly measures the activity of efflux transporters. ATP Binding Cassette or efflux transporters mediate the transport of substrates across cell membranes against a concentration gradient.

1 day ago · Luminescent assays measure viability based on ATP levels, a direct indicator of metabolically active cells. The ATP-based CellTiter-Glo assay employs a luciferase reaction to generate light proportional to ATP concentration. This assay offers exceptional sensitivity, detecting as few as 10 cells per well, making it ideal for low-cell-number ...