The bromodomain and extra-terminal motif (BET) protein BRD4 binds to acetylated histones at enhancers and promoters via its bromodomains (BDs) to regulate transcriptional elongation.

Aug 3, 2018 · BRD4-eRNA interactions increased BRD4 binding to acetylated histones in vitro and augmented BRD4 enhancer recruitment and transcriptional cofactor activities. Our results …

As BRD4 directly interacts with MYC and is involved in upregulation of MYC target gene, BRD4 may function with MYC to properly modulate RNA pol II initiation, topoisomerase function, …

Aug 17, 2023 · HiS-NET-seq uncovers new transcribed enhancers in human cells. A multi-omics analysis shows that genome-wide enhancer transcription depends on the BET family protein …

Dec 20, 2017 · Using two conditional knockout mouse strains and derived cells, we demonstrate that Brd4 controls cell identity gene induction and is essential for adipogenesis and …

BRD4 helps to assemble a functional Pol II elongation complex and recruits 3’-end RNA processing factors (CPSF, CstF) during a general 5’-elongation control point to allow …

Dec 14, 2012 · Transcriptional elongation by RNA polymerase II (Pol II) is regulated by positive transcription elongation factor b (P-TEFb) in association with bromodomain-containing protein …

The two bromodomains in BRD4, termed BD1 and BD2, consist of 4 alpha-helices linked by 2 loops. [9] The ET domain structure is made up of 3 alpha-helices and a loop. [10] The C …

Nov 21, 2024 · Bromodomain 4 (BRD4), a key regulator with pleiotropic functions, plays crucial roles in cancers and cellular stress responses. It exhibits dual functionality: chromatin-bound …

Nov 21, 2024 · Recently, the Shilatifard laboratory uncoupled BRD4’s N-terminal and C-terminal functions and demonstrated that the C-terminal domain is essential for releasing paused RNA …