The whole cell lysate input was probed using anti-FLAG antibody to determine the expression of flag-ELL2. GAPDH was detected as loading control.

Jan 15, 2019 · To understand if the decreased AGR2 by MG132 is caused by protein stability, cycloheximide (CHX), a protein synthesis inhibitor, was included in the study below.

Jan 4, 2011 · Inhibition of new protein synthesis by cycloheximide (CHX) can block the survival pathway and, thus, is widely used in studying cell death. Indeed, treatment of primary MEF cells with 30 ng/ml...

G IGF2BP1 levels in CNE-2 cells with HAX-1 knockdown or overexpression subjected to treatment with CHX or H MG-132 as determined by WB and quantitative analyses (GAPDH was used as the control). I IGF2BP1 expression in CNE-2 cells with HAX-1 knockdown or overexpression treated with CHX for the indicated time points as determined by WB and ...

GAPDH served as a loading control (n = 3, *p < 0.01). Following the treatment of CHX, the Ca v 1.2 protein level showed a time-dependent decrease. The UBR6 reduction-induced increase in Ca v 1.2...

Inhibition of protein synthesis induces phosphorylation of AKT substrates. a, upper panel, HEK-293FT cells were transfected with HA-WT AKT for 16 h, followed by treatment with 100 μ m cycloheximide (CHX) for 3 h. The levels and phosphorylation of AKT and its substrates were analyzed by Western blotting.

Jul 16, 1990 · GAPDH enzymatic activity is induced approx. sevenfold over resting levels. The induction of GAPDH mRNA is inhibited only slightly by CHX under conditions in which cell proliferation is inhibited.

Thank you, would i still use Actin or GAPDH as a control, will it be influenced by cycloheximide. You might want to also probe your blots for p53 or something similar with a short half-life to...

Sep 16, 2024 · In this study, we identified mitochondrial GAPDH as a new substrate of the E3 ubiquitin ligase Parkin, which mediated GAPDH ubiquitination in human cervical cancer.

May 9, 2023 · Cells were treated with 100 µg/mL cycloheximide (CHX) and were harvested at the indicated times after the addition of CHX. GAPDH was used as the internal loading control. H, Quantification of stability assays shown in G.