NPM1 induced the ubiquitin-mediated proteasome degradation of p53 by recruiting Mdm2. A, C. Cycloheximide (CHX) chase analysis of the half-life of p53 in control and NPM1-overexpressing 5-8F and HONE1 cells. CHX: 50 μg/ml. B, D. The half-life of p53 in 5-8F and HONE1 cells. E, F.

Oct 24, 2023 · Overexpression of PSMD2 significantly facilitated the degradation of endogenous MYH9 in HONE1 cells treated with CHX (Fig. 5E).

Feb 6, 2024 · HONE1 (middle) and SUNE1 (right) cells co-transfected with FLAG-ATMIN, HA-ubiquitin (Ub) and si-USP10#2 or si-NC were subjected to Co-IP and immunoblotted with the indicated antibodies.

Jul 8, 2024 · To investigate whether the G3BP1 K76R mutant would affect the stability of G3BP1, we conducted western blotting assay in HONE1 and SUNE1 cells that transfected with siRNA targeting G3BP1 (Figure S7A, Supporting Information) and then overexpressed with G3BP1- WT or K76R mutant following CHX treatment.

Restoration of Vimentin reverses the tumor suppressive effect of NRURL3. HONE1, SUNE1, and HK1 cells were transiently co-transfected with HA-NEURL3 or empty vector together with Flag-Vimentin or empty vector. (a) The migratory abilities of transfected NPC cells were determined by wound healing assay.

May 8, 2024 · In this study, using a soft fibrin gel culture system, we found that tumor-repopulating cells (TRCs) with stem-cell-like cancer cell characteristics resist chemotherapy and radiotherapy by...

Curcumin reduced the half-time of EBNA1 in HK1-EBV and HONE1 cells after CHX treatment compared with the control groups (Figures 4(f) and 4(g)). In addition, the effects of curcumin on the proteasome pathway were investigated.

Aug 15, 2018 · Importantly, EBNA1 was critical for NPC cells to resist caspase-9-dependent apoptosis induced by triptolide. Finally, we revealed that triptolide significantly inhibited the growth of xenografted tumor induced by HONE1-Akata cell in BALB/c nude mice.

Feb 10, 2025 · Subsequently, ALG3 was knocked down or over-expressed in HONE1 and C666-1 cells following treatment with cycloheximide (CHX), which revealing that ALG3 could strikingly enhance the protein stability of FOXD1 (Supplementary Fig. 12A).

Sep 1, 2024 · Our study revealed high overexpression of the lncRNA DYNLRB2-AS1 in GEM-resistant NPC cells and demonstrated that DYNLRB2-AS1 promoted NPC growth and GEM resistance via enhancing DHX9 expression.