A ubiquitin ligase (also called an E3 ubiquitin ligase) is a protein that recruits an E2 ubiquitin-conjugating enzyme that has been loaded with ubiquitin, recognizes a protein substrate, and …

Jul 30, 2021 · E3 ubiquitin ligases play an essential role in catalyzing the ubiquitination process and transferring ubiquitin protein to attach the lysine site of targeted substrates. Importantly, …

The E3 ligases of the HECT (homologous to the E6AP carboxyl terminus) domain family catalyze ubiquitin transfer to the substrate protein through a two-step reaction: ubiquitin is first …

Nov 23, 2023 · We present BioE3, a powerful approach for matching substrates to Ub E3 ligases of interest. Using BirA-E3 ligase fusions and bioUb, site-specific biotinylation of Ub-modified …

Mar 21, 2024 · Here we report the development of E3-substrate tagging by ubiquitin biotinylation (E-STUB), a ubiquitin-specific proximity labeling method that biotinylates ubiquitylated …

Oct 5, 2022 · Here, we report that DTX-family Ub E3 ligases ubiquitylate ADP-ribosylated proteins and peptides on the ADPr modification in vitro, producing a potential PTM that combines ADPr …

Apr 4, 2014 · Three distinct classes of E3 ligases have been identified that stimulate transfer of ubiquitin and ubiquitin-like proteins through either a direct or an indirect mechanism. Only …

E3 ligases are a critical component of the ubiquitin proteasome system (UPS), determining the substrate specificity of the cascade by the covalent attachment of ubiquitin to substrate …

(A) Schematics of E3 ubiquitin (Ub)-ligases in plants. RING (Really-Interesting New Gene) and U-box E3 ligases bind an E2 and the target and facilitate direct transfer of Ub to the target protein.

Jan 6, 2025 · We report that the outer mitochondrial membrane (OMM)-associated E3 Ub ligase MARCH5 is vital for generating mitochondria-derived pre-peroxisomes.