Avidin is a tetrameric biotin -binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. Dimeric members of the avidin family are also found in some bacteria. [1] . In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1800 μg per egg).

Sep 1, 1984 · Avidin conjugated to the fluorescent dyes rhodamine or fluorescein binds to mast cell granules in rodent and human skin. Sequential staining of tissue mast cells first with conjugated avidin, and then with a metachromatic stain demonstrated that both techniques identify the same mast cell granules.

Recently, the avidin-biotin binding system (ABBS) was used to fabricate PLCL/Pluronic nanofiber matrix for skin care application. Silk biomaterials with attached bioactive molecules are extensively used for drug delivery and tissue engineering.

The glycoprotein, avidin, conjugated either to the enzyme horseradish peroxidase, or to the fluorochrome dyes, fluorescein or rhodamine, identifies the granules of mast cells in both tissues and cell suspensions.

Avidin is a basic tetrameric glycoprotein composed of four identical subunits; each binds to biotin with high specificity and affinity (Kd ∼10−15 M). Avidin is originally derived from the eggs of reptiles and amphibians.

The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the ...

May 16, 2015 · PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area.

Biotin contributes to healthy skin and hair, and may play a role in preventing hair loss. Consuming raw eggs in large amounts over a prolonged period can contribute to biotin deficiency. This is not a problem when consuming cooked eggs, which are a good dietary source of biotin.

Safranin and fluorescent dyes: berberine and avidin — also bind to heparin. Longer staining with histological dyes or alcian blue staining is needed to label mucosal and immature mast cells. Advanced techniques — enzyme histochemistry and especially immunohistochemistry — enable to detect mast cells high-selectively using a reaction to ...

Avidin conjugated to the fluorescent dyes rhodamine or fluorescein binds to mast cell granules in rodent and human skin. Sequential staining of tissue mast cells first with conjugated avidin, and then with a metachromatic stain demonstrated that …