Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+). Sequence Author: MilliporeSigma …

There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, …

pET-28a (+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins …

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at …

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker. Midwest Center for Structural Genomics.

Plasmid pET-28a (+)::NL from Dr. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019. This …

Plasmid pET28a(+)/FaGH16A from Dr. Jan-Hendrik Hehemann's lab contains the insert FaGH16A and is published in Appl Environ Microbiol. 2017 Feb 17. pii: AEM.03389-16. doi: …

pET-28a(+) DNA - Novagen Novagen′s pET-28a-c(+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence.; …

Aug 16, 2011 · The pET-28a (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown …

We present improved designs and demonstrate that, when incorporated into pET28a, they support increases in protein production. The improved designs are applicable to most of the …

All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins. The pET-28a-c (+) vectors …

All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins. The pET-28a-c(+) vectors …

The pET-28a-c (+) vectors carry an N-terminal His Tag/thrombin/T7 Tag configuration plus an optional C-terminal His Tag sequence. The pET Vectors are supplied as purified plasmid DNA …

pET-28a (+) is a bacterial expression vector that is used for the expression of N-terminally 6xHis-tagged proteins with a thrombin site. The vector carries an N-terminal …

pET-28a is a commonly used fusion protein type prokaryotic high-efficient expression vector, which contains kanamycin resistance gene. Expression is induced by T7 RNA polymerase …

Briefly, genes were cloned into pET28a to generate an N-terminal His-tagged fusion protein. Expression of the CheA gene was induced by the addition of 0.1 mM IPTG for 5 h at 30°C, …

Dec 21, 2023 · SIR2 catalyzes the depletion of NAD + for anti-phage defense. SIR2-HerA, a bacterial two-protein anti-phage defense system, induces bacterial death by depleting NAD + …

Dec 12, 2024 · Human TRIM21 PRYSPRY (residues 287-465) was cloned into the pET28a vector with an N-terminal 6xHis tag. The D355A mutation was introduced by overlap extension PCR. …

Here, we present a biochemical and structural analysis of E. coli HerA (Ec HerA), SIR2 (Ec SIR2), and the SIR2-HerA complex, revealing a unique mechanism of assembly-mediated activation …