Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse applications such as gene expression analysis, the detection of genetically modified organisms in food, and cancer phenotyping.

A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.

Learn to design, optimize, and validate real-time PCR assays. Primer and probe design, target selection, gradient, melt curve, and multiplexing for qPCR assays.

Quantitative reverse transcription polymerase chain reaction, also called RT-qPCR, is used to detect and quantify RNA. Total RNA or mRNA is first transcribed into complementary DNA (cDNA). The cDNA is then used as the template for the quantitative PCR or real-time PCR reaction (qPCR).

Real-time quantitative polymerase-chain-reaction (qPCR) is a standard technique in most research laboratories performing gene expression. qPCR data analysis is a crucial part of a gene expression experiment, and has led to the development of several key methods.

A general overview of the basics of real-time PCR (qPCR) assays, sequence detection chemistries, TaqMan and SYBR chemistries, quantitation assays, and methods of absolute vs. relative quantitation.

Jun 15, 2013 · Review these definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments. In addition, this article directs you to many valuable resources to explain qPCR assay design, components, and applications.

Quantitative PCR (qPCR) is one of the foremost methods for detection and quantification of nucleic acid targets. The technique is also known as real-time qPCR and is not to be confused with RT-qPCR (Reverse Transcription-qPCR).

Real-time PCR, also known as quantitative or qPCR, determines the actual amount of PCR product present at a given cycle. By using a fluorescent report in the PCR reaction, this process allows you to measure DNA generation in the qPCR assay. There are two methods of qPCR: SYBR Green and probe-based. SYBR Green-based q PCR

4 days ago · Plate format also affects volume selection, with 384-well plates enabling high-throughput analysis and 96-well plates offering greater assay flexibility. Standard Curve Generation. Accurate quantification in RT-qPCR requires a standard curve to establish the relationship between cycle threshold (Ct) values and known template concentrations.