Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. Dimeric members of the avidin family are also found in some bacteria. [1] In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1800 μg per egg).

Apr 23, 1993 · Three-dimensional structures of avidin and the avidin-biotin complex. The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively.

The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the ...

Jun 1, 1993 · The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the tetrameric assembly and for the exceptionally tight …

Structure. As noted, avidin forms an eight-stranded b barrel. Each strand is connected to the next by an extended loop of four to twelve amino acid residues. The strands are antiparallel and are characterized by a right-handed twist. Active avidin exists as a …

Here, we assess the ability of tandem-trapped ion mobility spectrometry – mass spectrometry (tandem-TIMS/MS) to characterize the structure and sequence of the homo-tetrameric glycoprotein avidin.

The chicken genome encodes several biotin-binding proteins, including avidin and avidin-related protein 4 (AVR4). In addition to D-biotin, avidin binds an azo dye compound, 4-hydroxyazobenzene-2-carboxylic acid (HABA), but the HABA-binding properties of …

Jun 1, 1993 · Three-dimensional structures of avidin and the avidin-biotin complex. The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively.

Three-dimensional structures of avidin and the avidin-biotin complex. with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the tetrameric assembly and for the exception- ally tight binding ofbiotin.

Avidin(Av) is a protein found in egg white which binds biotin (vitamin B7) with high affinity. This property makes it a powerful tool in various protein pull-down assays. Similar proteins are: AVR2 and AVR4 are avidin-related proteins.